Clinical trial

Obesity and Aging: Impacts of Nutritional Counseling and Physical Exercise on Telomeres and Senescence Parameters

Active, not recruiting · Not applicable · 1 countries · Registry ID NCT07479940

Active, not recruitingNot applicableInterventional

What this study is about

The study will involve 80 elderly women, 40 eutrophic and 40 obese, divided into four groups, each with n=20. 1) Eutrophic Group (EG) undergoing only nutritional counseling (NC), 2) Eutrophic Group with NC and combined training (CT), 3) Obese Group (OG) undergoing only NC, and 4) Obese Group with NC and CT. The study will be divided into two main phases: an initial 9-week phase dedicated to NC, followed by a 20-week CT phase.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age60 Years to 75 Years

SexFemale

Healthy volunteersAccepted

ConditionAging, Obesity, Nutrition, Exercise, Telomere Shortening, Inflammation, Oxidative Stress, Oxidative DNA Damage

Full registry criteria

Inclusion Criteria * being female; * between 60 and 75 years old; * a BMI between 22 and 40 kg/m²; * being sedentary (not exercising for at least 3 months). Exclusion Criteria: * a history of cancer, autoimmune or neurodegenerative diseases; * excessive alcohol consumption; * smoking * score ≤13 on the Mini-Mental State Examination (MMSE).

Treatments and study arms

Combined Training

Other

20 weeks of 60-minute combined training sessions (30 minutes of aerobic training and 30 minutes of strength training) three times per week

Nutritional Counseling

Other

9 weeks of nutritional counseling with the purpose of teaching about essential macronutrients and their functions, encouraging adequate protein and fresh food consumption

Primary outcomes

Telomere length in peripheral blood mononuclear cells (PBMCs) measured by quantitative PCR9 and 29 weeks

To evaluate the effect of obesity, nutritional counseling, and combined physical training on leukocyte telomere length in elderly women. Telomere length will be measured in peripheral blood mononuclear cells (PBMCs) using quantitative real-time PCR (qPCR), expressed as the telomere-to-single copy gene (T/S) ratio according to the Cawthon method.

Expression of cellular senescence-related genes in PBMCs measured by quantitative RT-PCR9 and 29 weeks

To assess the effect of obesity, nutritional counseling, and combined physical training on the expression of genes related to cellular senescence in peripheral blood mononuclear cells (PBMCs). Gene expression will be quantified by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and normalized to a housekeeping gene.

Adequacy of protein intake assessed by dietary intake analysis0, 9 and 29 weeks

To evaluate the effect of nutritional counseling on protein intake adequacy in elderly women. Dietary intake will be assessed using 24-hour dietary recalls, and protein intake will be calculated using nutritional analysis software. Adequacy will be determined by comparing individual protein intake with recommended intake values for older adults.

Percentage of ultra-processed food consumption assessed by NOVA food classification0, 9 and 29 weeks

To evaluate the effect of nutritional counseling on the consumption of ultra-processed foods in elderly women. Dietary intake will be assessed using 24-hour dietary recalls. Foods will be classified according to the NOVA food classification system, and the proportion of total energy intake derived from ultra-processed foods will be calculated.

Diet quality assessed by proportion of ultra-processed food consumption according to the NOVA classification0, 9 and 29 weeks

To assess the effect of nutritional counseling on diet quality in elderly women based on the degree of food processing. Dietary intake will be evaluated using 24-hour dietary recalls, and foods will be classified according to the NOVA classification system. Diet quality will be determined by calculating the proportion of total energy intake derived from ultra-processed foods, with higher values indicating poorer diet quality

Study locations

1 locations were listed when this page was built. The first 40 are shown.

Universidade de São Paulo - Escola de Educação Fisica e Esporte🇧🇷 Ribeirão Preto, São Paulo, Brazil

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