Clinical trial

Long-term Adaptations of Skeletal Muscle After Hybrid Training

Recruiting now · Not applicable · 1 countries · Registry ID NCT07341711

Recruiting nowNot applicableInterventional

What this study is about

Obesity is a major challenge for public health and renders it imperative to reduce its prevalence. High intensity interval training (HIIT) is a form of exercise training that can efficiently induce weight loss in adults with overweight or obesity, even in the absence of dietary intake manipulation. Hybrid type training represents a form of HIIT, that incorporates both cardiorespiratory and musculoskeletal stimuli, by combining multiple types of exercise into a circuit-type, interval style workout. Recent evidence suggests that long-term participation in hybrid HIIT results in significant health-related benefits. However, the molecular mechanisms driving the chronic effects of hybrid HIIT on cardiometabolic and musculoskeletal health remains to be elucidated.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age30 Years to 50 Years

SexAll

Healthy volunteersAccepted

ConditionObesity & Overweight

Full registry criteria

Inclusion Criteria: * BMI: \>25 kg/m2 and \<40 kg/m2 * Untrained individuals (abstain \>1 year from exercise training) * No dietary intervention over the last 6 months preceding the study * Low cardiorespiratory fitness level (VO2max: \<45 ml/kg/min) * No use of any medication, dietary supplements * Low risk for cardiovascular disease * A weight loss \<10% over the last 6 months preceding the study * Free of non-communicable diseases (excluding metabolic syndrome) Exclusion Criteria: * Low participation rate (\<80% completion of the exercise training sessions) * Unbalanced diet * Participation in additional exercise training regimes * Cosumption of anti-inflammatory of pain relief medication

Treatments and study arms

Exercise training

Other

Participants will perform a six-month hybrid training program while receiving a balanced diet. The periodization of hybrid training intervention will consist of three 2-month phases of gradually increased exercise intensity and volume. In every training will participate 5-8 individuals. The training will contain 6-12 different exercises (stations), depending on the phase of the intervention, which will be executed in a circuit for a total of 2-3 rounds, with 2-3 minutes of rest period between sets (depending on the phase). The exercise execution will last 20-45 seconds, and the rest between them will last 30-60 seconds (depending on the phase), while the exercise intensity will range from 75 to 85% of maximal heart rate. The stations of hybrid training will contain multi-joint exercises or neuromuscular activation exercises using either body weight resistance or portable equipment.

Control group

Other

Participants will receive a balanced diet but will not participate in any type of exercise training over a six month period.

Primary outcomes

Change in mitochondrial sizeAt baseline and at 6 months

Mitochondrial size will be measured using transmission electron microscope

Change in mitochondrial densityAt baseline and at 6 months

Mitochondrial density will be measured using transmission electron microscope

Change in mitochondrial countAt baseline and at 6 months

Mitochondrial count will be determined using transmission electron microscope

Change in mitochondrial distributionAt baseline and at 6 months

Mitochondrial distribution will be determined using transmission electron microscope

Change in maximum oxygen consumption (VO2max)At baseline and at 6 months

Maximum oxygen consumption (VO2max) will be assessed during a cardiopulmonary exercise testing by using a portable indirect calorimetry system

Change in muscle fiber cross-sectional areaAt baseline and at 6 months

Muscle fiber cross-sectional area (μm2) will be measured using immunohistochemical staining for myosin heavy chain

Change in PAX7+ satellite cells countAt baseline and at 6 months

PAX7+ satellite cells will be determined using immunohistochemistry techniques.

Change in total protein contentAt baseline and at 6 months

Total protein content (total RNA) will be determined in skeletal muscle tissue using real time quantitative-Polymerase Chain Reaction (q-PCR) technique

Study locations

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Department of Physical Education and Sport Science Trikala, University of Thessaly🇬🇷 Trikala, Thessaly, Greece

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Ioannis G Fatouros, ProfessorContact

Email · Call +30 2431047047

Athanasios Z Jamurtas, ProfessorContact

Email · Call +30 2431047054