Clinical trial

Upper Body Subcutaneous Exosome Release in Response to a Meal in Obesity

Recruiting now · Not applicable · 1 countries · Registry ID NCT07031297

Recruiting nowNot applicableInterventional

What this study is about

The purpose of this study is to understand the specific outflow and difference between normal weight and volunteers with obesity of upper body subcutaneous adipose tissue in response to a mixed meal challenge by site specific cannulation of the superior epigastric vein. We will characterize the exosome signatures in response to a meal and compare to the exosome signature of arterialized venous plasma samples.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age18 Years to 55 Years
SexAll
Healthy volunteersAccepted
ConditionObesity

Full registry criteria

Inclusion Criteria: • All women must be premenopausal Exclusion Criteria: * Participants taking medications know to affect blood flow (statins, β-blockers) or fatty acid or adipose tissue metabolism (TZDs, high dose fish oil supplements) * Diabetes, history of cardiovascular disease * Allergy to lidocaine * Post-menopausal women * Pregnant

Treatments and study arms

Meal Study

Diagnostic Test

Participants will consume a mixed meal drink (Ensure) equivalent to 40% of basil metabolic rate as calculated via Harris Benedict equation. Following consumption of the drink they will undergo a series of blood draws over the following 4 hours: * Plasma glucose will be measured according to a mixed meal test protocol every 10 minutes for the first hour and then every 15, then 30 minutes for the last 3 hours. * 1 ml plasma aliquots for exosome analysis and insulin concentrations will be drawn every 30 minutes following consumption of ensure for 4 hours. * Blood gasses will be drawn every 30 minutes for the 4 hours following the meal. Participants will undergo a second abdominal adipose tissue biopsy 3 hours following mixed meal drink.

Primary outcomes

Adipose tissue blood flow4 hours

Blood flow will be measured in the upper body subcutaneous adipose tissue using Xenon washout and reported as blood flow in ml/min/100g adipose tissue.

Study locations

1 locations were listed when this page was built. The first 40 are shown.

Mayo Clinic in Rochester🇺🇸 Rochester, Minnesota, United States
Kelli A LytlePrincipal Investigator