Clinical trial

Stable Isotopes- Adults With Obesity

Recruiting now · Not applicable · 1 countries · Registry ID NCT07020741

Recruiting nowNot applicableInterventional

What this study is about

In a crossover design, 8 participants will receive caloric drinks containing stable isotopes. In one arm of the study, participants will consume 6 hourly drinks containing two stable isotopes. Repeated blood, breath, urine, and muscle biopsies will be taken. In the second arm, participants will consume 1 drink containing 75g glucose, labeled with two stable isotopes of glucose. Periodic blood and breath will be taken over three hours.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age19 Years to 59 Years

SexAll

Healthy volunteersAccepted

ConditionObese Patients (BMI ≥ 30 kg/m²), Glucose Metabolism, Protein Metabolism

Full registry criteria

Inclusion Criteria: * ages 19-59 * Obese \>/= 30 kg/m\^2 and \>/= 102 cm for males and \>/= 88 cm for females * Godin Leisure-time or exercise questionnaire \<14 units Exclusion Criteria: 1. Pregnant or become pregnant 2. Any known food allergies 3. Smoker 4. Are physically active 5. Have any known metabolic diseases 6. Have been weight unstable within the last 6 months (lost or gained \>10% of body mass) 7. Non eumenorrheic or on a hormonal birth control

Treatments and study arms

Indispensable Amino Acid Oxidation

Procedure

Participants will ingest six hourly meals designed to provide 50% of the daily intake of 1.1 g·kgLBM. Additional energy (1.5x daily resting metabolic rate), carbohydrate (55%), and fat (35%) will be provided in beverages and protein-free cookies (for palatability). The first drink will contain priming doses of NaH13CO2 (0.176 mg·kg-1), \[13C\]phenylalanine (1.86 mg·kg-1), \[2H5\]phenylalanine (0.34 mg·kg-1) with all subsequent drinks containing 1.2 mg·kg-1 \[13C\]phenylalanine and 0.51 mg·kg-1 \[2H5\]phenylalanine.

Stable Isotope Oral Glucose Tolerance Test

Procedure

Participants will consume a 75g glucose drink dissolved in 250 ml of water enriched with \[6,6\_2H2\]glucose and \[13C6\]glucose.

Primary outcomes

Define obesity-related whole body protein efficiency based of the IAAO method0 - 6 hours observational period ingesting six hourly meals, within 1 week of preliminary testing. Baseline measures taken at --30 minutes.

Amino Acid Oxidation of phenylalanine will be measured using the IAAO method by periodic ingestion of amino acid mixture. Trials will start at \~7:00 am (-30 minutes) with a total duration of 6.5 hours (390 minutes) Measurements of breath (-30, 0, 120, 240, 270, 300, 330 and 360 minutes), Urine (-30, 0, 240, 300, and 360 minutes), muscle biopsy (-30 and 360 minutes) and blood (-30, 0, 30, 60, 90,120, 180, 240, 300, and 360 minutes) will be used to determine the whole body efficiency of protein in obese populations.

Assessment of oral glucose tolerance based on a dual tracer technique0- 3 hour observational period. Within 1 week of the prospective arm. Baseline taken at -30 minutes.

Measure glucose kinetics via \[13C\] and D5 Glucose over 3 hours to determine glucose tolerance. Trials will start at \~7:00 am (-10 minutes) and last \~ 3 hours. (190 minutes)

Study locations

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Freer Hall - University of Illinois🇺🇸 Urbana, Illinois, United States

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Nicholas Burd (Associate Professor), PhDContact

Email · Call 2172440970

Gena L Irwin Research Assistant, M.SContact

Email · Call 3608883281

Nicholas Burd, PhDPrincipal Investigator