Clinical trial

Stem Cell Isolation From Femoral Heads Post Total Hip Arthroplasty

Opening soon · Not applicable · 0 countries · Registry ID NCT06775600

Opening soonNot applicableInterventional

What this study is about

Femoral heads will be collected at University Hospital (London, ON, Canada) from individuals with type-2 diabetes, obesity, and no T2D/no obesity controls. The femoral head will be taken to the Robarts Research Institute (London, ON, Canada) for lineage-restricted progenitor cell expansion and analyses. There the femoral heads will be used to isolate three stem cell types - Multipotent Stromal Cells, Endothelial Colony Forming Cells, and Hematopoietic Progenitor Cells. The yield and cell surface markers expressed on these cells will be measured using flow cytometry. These cell types will then undergo further analyses of the secretome, tubule forming assays, vascular regenerative cell exhaustion assays, and will be transplanted into immune deficient mice with with femoral artery ligation. These experiments are to assess the differences in function and expression of these cell types between individuals with type-2 diabetes and/or obesity and/or healthy controls.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age18 Years to Not listed

SexAll

Healthy volunteersNot accepted

ConditionObesity and Type 2 Diabetes

Full registry criteria

Inclusion Criteria: * Must be 18+ years of age Exclusion Criteria: * Diagnosis of bone cancers * Diagnosis of Rheumatoid arthritis * Diagnosis of hematopoietic conditions * Diagnosis of avascular necrosis of the hip * does not fall within BMI requirements for experimental groups * Cannot read and/or speak in english

Treatments and study arms

Hip Replacement

Procedure

Total Hip Arthroplasty where the Femoral Head will be used to harvest stem cells post-extrication from the individual.

Primary outcomes

Cell YieldWithin 24 hours of surgery

Quantification of how many cells are harvested from each femoral head prior to expansion in vitro

Cellular Growth KineticsTime of cell harvest to 8 weeks post harvest

Quantification of the Growth Kinetics of each cell type during in vitro cell culture post cell harvest.

Cellular PhenotypeTime of cell harvest to 8 weeks post harvest

Analysis via flow cytometry of the phenotype of each cell type and their respective surface markers that are involved in regenerative pathways.

Study locations

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No public site location was included in this record. Check the original registry for updates.