Clinical trial

How a Single Workout Affects Gut Bugs in Women With Different Fitness Levels and Body Types

Recruiting now · Not applicable · 1 countries · Registry ID NCT06691100

Recruiting nowNot applicableInterventional

What this study is about

This study aims to elucidate the differences in the gut microbiome functional activity and metabolome in adult premenopausal women with distinctive fitness levels and BMIs (with obesity, w/o obesity). The specific aims are as follows: * Aim 1: To examine the effects of acute aerobic exercise at 60-70% heart rate reserve (HRRmax) for 30 minutes bout on changes in the abundance of SCFA-producing bacteria and their functional downstream metabolic activity. * Aim 2: To examine the effects of acute aerobic exercise at 60-70% HRRmax 30-minute bout on changes in GM-released SCFA concentrations in stool and plasmatic metabolome.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age21 Years to 40 Years

SexFemale

Healthy volunteersAccepted

ConditionWeight Management, Obesity, Exercise, Women, Gut Microbiome, Metabolome, Health, Gut Microbiota

Full registry criteria

Inclusion Criteria: * Biological sex: female * BMI 19 to 24.99 kg/m2 or BMI 30-40 kg/m2 * Exercisers: Exercise at least 4 times per week for 60 minutes (a total of 240 minutes per week) on a programmed exercise training either on endurance sports (e.g., running, cycling, triathlon), strength (e.g., powerlifting), or team sports (e.g., rugby, football, soccer) * Non-Exercisers: Sedentary individuals who have not reached the PA guidelines (150 minutes of moderate-intensity physical activity a week, 75 minutes of vigorous-intensity physical activity, or an equivalent combination of moderate and vigorous-intensity physical activity). Exclusion Criteria: * Biological sex: male * BMI \< 19 kg/m2 or \> 40 kg/m2 * Currently pregnant, \< 2 years postpartum, lactating * Currently taking any herbal, fiber, or prebiotic supplement * Current or 1-month before the study, taking oral or vaginal antibiotics * Diagnosed with any gastrointestinal, endocrine, digestive, cancer, or cardiovascular disease.

Treatments and study arms

Aerobic Exercise

Behavioral

30-minute exercise bout in a cycle ergometer at 60 to 70% of the Heart Rate Reserve (HRR) and corresponding power (watts).

Primary outcomes

Gut Microbiome CompositionBefore the exercise intervention and immediately after the intervention

Shotgun metagenomics will be used to examine the abundance of specific SCFA-producing bacteria in the stool (e.g., Ruminococcus)

Gut Microbiome FunctionBefore the exercise intervention and immediately after the intervention

Shotgun metagenomics will be used to examine the BGCs (i.e., the genes within the microbial genome that dictate gut microbial functionality) to investigate functional downstream metabolic activity.

Plasma MetabolomeBefore the exercise intervention, immediately after the exercise intervention, and 24 hours before the intervention

Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in plasma before and after the exercise intervention

Stool MetabolomeBefore the exercise intervention, immediately after the exercise intervention, and 24 hours before the intervention

Gas chromatography and mass spectrometry will be used to measure concentrations of SCFAs in stool before and after the exercise intervention

Study locations

1 locations were listed when this page was built. The first 40 are shown.

Milken Institute School of Public Health, The George Washington University🇺🇸 Washington D.C., District of Columbia, United States

Open map

Carmen Ortega-Santos, PhD, RDContact

Email · Call 202-994-2757