Clinical trial

Effect of Weight Loss on Intermuscular Adipose Tissue (IMAT) Signaling

Recruiting now · Not applicable · 1 countries · Registry ID NCT06129110

Recruiting nowNot applicableInterventional

What this study is about

The goal of this intervention study is to learn about how weight loss impacts molecular signaling of intermuscular adipose tissue (IMAT) in individuals with obesity. The main question it aims to answer is how inflammatory molecules secreted by IMAT promote muscle insulin resistance and inflammation, and how these same molecules are diminished after weight loss. Following screening visits involving body composition measures, blood testing, strength testing, and a thigh muscle biopsy, participants will go through a 12-week dietary intervention for weight loss. After 12 weeks, this will be followed by the same testing and biopsies that were completed before the intervention. Researchers will then compare outcomes of individuals who lost weight to individuals who did not lose weight.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age18 Years to 70 Years

SexAll

Healthy volunteersAccepted

ConditionObesity, Insulin Sensitivity, Muscle Weakness, Adiposity, Insulin Resistance

Full registry criteria

Inclusion Criteria: * Generally healthy men and women aged 18-70 * BMI between 30-40 * Less than 1 hour of exercise per week * Women: 1. may be pre or post menopausal Exclusion Criteria: * Type 1 or Type 2 diabetes * Thyroid disease * History of lung disease * Active use of nicotine * Severe plasma lipid disorders * Taking hormone replacement drugs, blood thinners, or thiazoladinediones * Women: 1. Currently going through menopause or peri-menopause 2. Pregnant or breastfeeding 3. History of Polycystic Ovary Syndrome

Treatments and study arms

Diet Weight Loss

Behavioral

Low calorie diet of meal replacement shakes

Primary outcomes

Insulin Sensitivity12 weeks

Participants will undergo a standard 3-hour hyperinsulinemic/euglycemic clamp with insulin infused at 80 mU/m2/min, and glucose clamped at 90 mg/dl with a variable dextrose infusion.

Body Weight12 weeks

Participant weight will be measured using BodyTrace scales during the 12 week intervention.

IMAT Content12 weeks

A multi-slice MRI will be used to quantify IMAT and muscle content in both legs with acquisition of \~20 axial images (10 mm thickness) with 10 mm spacing between images starting superior to the patella. IMAT content will be quantified using the well-validated Sirlin 6-echo method and normalized to muscle volume.

Muscle Mass12 weeks

A multi-slice MRI will be used to quantify IMAT and muscle content in both legs with acquisition of \~20 axial images (10 mm thickness) with 10 mm spacing between images starting superior to the patella. Muscle volume will be quantified using standard methods with anatomical cross-sectional area evaluated in each T1 weighted, high resolution, gradient echo profile scan, and multiplied by the length of the muscle.

Muscle Strength12 weeks

Participants will perform quadriceps muscle strength testing using an isokinetic dynamometer.

Study locations

1 locations were listed when this page was built. The first 40 are shown.

University of Colorado Anschutz🇺🇸 Aurora, Colorado, United States

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Bryan Bergman, PhDContact

Bryan Bergman, PhDPrincipal Investigator