Clinical trial

A Study of Adipose Tissue in Adaptive Responses to Exercise

Active, not recruiting · Not applicable · 1 countries · Registry ID NCT06053125

Active, not recruitingNot applicableInterventional

What this study is about

The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age18 Years to 85 Years

SexAll

Healthy volunteersAccepted

ConditionHealthy, Obesity

Full registry criteria

Inclusion Criteria: * Adults between the ages of 18-35 OR 65-85. * BMI 18.5 kg/m2 - 40 kg/m2 * Weight stable (≥ 3 months) * Generally healthy Exclusion Criteria: * Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week. * Smoking/tobacco use. * Alcohol/substance abuse. * Pregnancy and breastfeeding. * Anemia. * Abnormal renal function. * Blood clotting disorders. * Coronary artery disease. * Uncontrolled thyroid disease. * Liver disease. * Use of medications known to influence the main outcomes of the study. * Orthopedic problems that may be aggravated by exercise. * Chronic disease at the discretion of the investigators.

Treatments and study arms

Adipose Tissue Biopsy

Procedure

Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise

Exercise

Behavioral

30 minutes of exercise on a cycle ergometer exercise machine

Primary outcomes

Adipose lymphocyte populationsPre-exercise, immediately post-exercise, and 3 hours after exercise

The numbers of lymphocytes in the adipose tissue will be assessed by flow cytometry.

IL6 secreted from adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)Pre-exercise, immediately post-exercise, and 3 hours after exercise

The concentration of 96 IL6 will be measured using the targeted inflammation Olink proximity extension assay panel in media obtained from cultured adipose tissue explants. Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed for the assessment of secreted inflammatory proteins. T

Adipose tissue macrophage populationsPre-exercise, immediately post-exercise, and 3 hours after exercise

Numbers of macrophages in the adipose tissue will be assessed by immunohistochemistry.

Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR).Pre-exercise, immediately post-exercise, and 3 hours after exercise

Gene expression of the inflammatory cytokine IL-6 will be measured in adipose tissue samples using real time (RT)-PCR.

Adipose tissue gene expression of interleukin (IL)-8 measured by PCR.Pre-exercise, immediately post-exercise, and 3 hours after exercise

Gene expression of the inflammatory cytokine IL-8 will be assessed in adipose tissue samples using real-time (RT)-PCR.

Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR.Pre-exercise, immediately post-exercise, and 3 hours after exercise

Gene expression of the macrophage marker CD68 be assessed in adipose tissue samples using real time (RT)-PCR.

Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR.Pre-exercise, immediately post-exercise, and 3 hours after exercise

Gene expression of the anti-inflammatory macrophage marker CD163 will be assessed in adipose tissue samples using RT-PCR.

Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR.Pre-exercise, immediately post-exercise, and 3 hours after exercise

Gene expression of the anti-inflammatory macrophage marker CD206 will be assessed in adipose tissue samples using RT-PCR.

Study locations

1 locations were listed when this page was built. The first 40 are shown.

Mayo Clinic🇺🇸 Rochester, Minnesota, United States

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