Energy Metabolism Profiles Over Weight-loss and Eating Responses

Inclusion Criteria: * 20-70 years of age, inclusive * BMI ≥ 24kg/m2 * Not in other clinical studies currently or in the past three months Exclusion Criteria: * Fasting glucose \>7.0mmol/L or diagnosed diabetes or taking insulin or other blood glucose-lowering drugs * Blood pressure \> 160/100 mmHg; diagnosed phase II or III hypertension or cannot decrease SBP under 160mmHg after anti-hypertension drugs * Fasting blood TG≥ 5.7 mmol/L or fasting LDL-C≥ 4.9 mmol/L or cannot control TG \< 5.7 mmol/L or LDL-C \< 4.9 mmol/L after taking lipid lowering drugs * Pregnant or lactating * Attempting to change body weight in the past 3 months * Use of antibiotic in the preceding 3 months for 3-serial days * Use of estrogen therapy or hormonal drugs in the preceding 6 months * Smokers * History of alcohol abuse or other substance abuse (Alcohol abuse is defined as regular alcohol consumption \> 40 g/day for females or \> 80 g/day for males) * Severe renal disease or liver disease * Severe gastrointestinal diseases * Surgical events preceding 1 year (except appendicitis or hernia surgery) * Severe cardiovascular or cerebrovascular diseases * Implantation of heart stent or any device containing metal material * Cancer or receiving radiotherapy and chemotherapy within 5 years * Hyperthyroidism or hypothyroidism * Suffering from AIDS, hepatitis A, hepatitis B and other infectious diseases * Claustrophobia * Physical disability * Any mental disorders or current use of antidepressants * Cognitive disability