Clinical trial

Metabolic Inflexibility is Related to Elevated Muscle Anaerobic Glycolysis

Recruiting now · Not applicable · 1 countries · Registry ID NCT04320264

Recruiting nowNot applicableObservational

What this study is about

The focus of this proposal is on overweight (25\>BMI\<30 kg/m2) subjects, as these individuals exhibit a high risk of becoming obese and/or developing metabolic diseases. We hypothesize that in some overweight individuals there is a "metabolic program" in skeletal muscle which predisposes them to the development of obesity. Findings may lead to clinical screening tools for determining risk for obesity in non-obese individuals and targeting this group for prevention.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age18 Years to 50 Years

SexAll

Healthy volunteersNot accepted

ConditionMetabolic Inflexibility

Full registry criteria

Inclusion Criteria: * 18-50 years old * BMI of 25 - 30 kg/m2 * BMI \> 40kg/m2 * Lactate levels in top and lower 25% Exclusion Criteria: * Pregnant women * Mentally disabled * Prisoners * Smokers * Subjects with heart disease * Type 1 and 2 diabetes * Endocrine disease * Hypertension * Musculoskeletal disease * Peripheral occlusion * Hepatic disease * Have had weight fluctuations exceeding + 3% in the previous 12 months * On medications which alter carbohydrate metabolism will not be studied

Treatments and study arms

Weight loss surgery

Procedure

Severely obese women (BMI \>40) undergo sleeve gastrectomy

Primary outcomes

Carbohydrate/fat oxidation (RER) in the fasting condition.Years 1-5

RER will be measured from indirect calorimetry

Muscle oxygen consumption (substrate oxidation) in the fasting condition.Years 1-5

Muscle oxygen consumption will be measured by near-infrared spectroscopy (NIRS)

Muscle lactate release in the fasting condition.Years 1-5

Muscle lactate release will be measured by microdialysis

Change in Carbohydrate/fat oxidation (RER) in response to glucose + insulin (metabolic flexibility)Years 1-5

Indirect calorimetry during glucose clamp

Change in muscle fat oxidation in response to high fat feeding (metabolic flexibility).Years 1-5

Oxidation of fatty acid in muscle homogenate.

Change in muscle oxygen consumption (substrate oxidation) in response to high fat feeding (metabolic flexibility).Years 1-5

Oxygen consumption measured by near-infrared spectroscopy (NIRS)

Study locations

1 locations were listed when this page was built. The first 40 are shown.

East Carolina University🇺🇸 Greenville, North Carolina, United States

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Terry Jones, PhDContact

Email · Call 2527446249

Nicholas Broskey, PhDContact

Email · Call 2527374684