Clinical trial

Impact of Weight Loss on the Human Sperm Epitranscriptome

Active, not recruiting · Not applicable · 1 countries · Registry ID NCT03962699

Active, not recruitingNot applicableObservational

What this study is about

Increasing evidence suggests that non-communicable diseases such as in particular obesity and its associated metabolic diseases are inherited from parents to children throughout several generations by epigenetic mechanisms. Thus, this environmental stress would induce epigenetic modification in the germ line that once transmitted and maintained in the progeny would induce the development of the parental pathologies. Considering the increasing prevalence of these pathologies worldwide, we urgently need to understand this process in human. Based on published and unpublished data demonstrating that sperm RNAs are vectors of epigenetic inheritance of obesity mouse model, the investigative team hypothesizes that epitranscriptome of obese men play a central role in the paternal epigenetic inheritance of obesity and its associated metabolic diseases as epigenetic vectors in this process. To validate this hypothesis, the investigative team will use sperm from non-obese and obese men taken before and after surgery weight loss. Thanks to these cohorts, they propose to: (i) compare the epitranscriptome profiles of non-obese and obese men to identify the RNAs molecules which will be either qualitatively or quantitatively epigenetically modulated by obesity; (ii) compare the epitranscriptome profiles of obese men before and after surgery-weight loss to assess the reversibility of the newly acquired RNA modifications. Giving some answers to this central question will provide not only some clues about the molecular mechanisms involved in this process, elements which might be crucial to stop the spread of this disorder, but will also allow the identification of obese-susceptibility loci which expression may be modulate by environmental factors and consequently able to transmit the disease.

A promising-looking record is not the same as confirmed eligibility. The study team must review the full criteria and current recruitment status.

Basic eligibility

Age20 Years to 50 Years

SexMale

Healthy volunteersAccepted

ConditionObesity

Full registry criteria

Inclusion Criteria: * exhibiting a normal BMI from 18 to 25 * aged between 20-50 years * selected as fertile men in the context of a a medical consultation in Reproduction Biology laboratories * does not show their opposition to participate in the research study * presented no difficulties in oral and written comprehension of french language.

Treatments and study arms

The registry does not list a named intervention.

Primary outcomes

Impact of weight loss on sperm transcriptome of obese menBetween day 0 and day 365: collection of sperm samples from obese men before bariatric surgery. Between day 370 and 735: collection of the sperm sample from obese men after bariatric surgery. Day 740-800; RNA-seq and data analysis

To evaluate the impact of weight loss on sperm transcriptome, sperm RNA from obese men will be analyzed by RNA-seq before and one year after bariatric surgery. The supplied samples will be sequenced on the Illumina NextSeq500 and the resulting RNA-Seq Data will be bioinformatically analyzed.

Study locations

1 locations were listed when this page was built. The first 40 are shown.

Hopital Archet🇫🇷 Nice, France

Open map